Assuming anyone who has good experience doing limited proteolysis would have known what to use. Our major concern is common sample prep methods with any filters/cartridges will trap peptides of interest. So is there a LC column that can be used to bypass sample prep?
A quick look at the column specs (attached) suggests yes, it would be fine. A major factor when deciding if a column can be used for proteins is the pore size. Generally speaking ≥300Å and it's okay for proteins. Since the column you cite is "optimised for separating peptides and proteins" then you should not have an issues.
Thanks Peter for the info. I'd think it's fine with proteins, but still wonder if it's a good choice for unknown peptides.
Literature showed that the RAM direct injection column could be used. Does anyone have good experience using the following columns?
http://www.registech.com/Library/Catalog/RAM_2008.pdf
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