Different primers for same gene but different results in expression?

Hey everyone, I am currently doing my doctoral thesis at the department of pathology. I designed two different primers for my gene of interest having two different amplification sizes (130bp and...

17 February 2021 6,684 3 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

How to address SRMR (0.2 to 0.8) to make model fit?

Dear researchers, I want to update my knowledge regarding data analysis. I will be honored if you can help me to reduce in correcting SRMR in SEM_PLS. All the measures (AVE, CR, VIF, CA, HTMT) are...

09 February 2021 1,702 1 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Are ther ways to quantify / measure the degree of loneliness?

Are there ways to quantify / measure / monitor the degree of loneliness of team members in a Covid-19 lockdown teleworking situation? Team dynamics over a longer period of teleworking and...

16 November 2020 4,212 9 View

Does anybody have experiences with FFF separation of PHB particles?

I am trying to separate suspension of PHB particles, stabilized with PVA. The DLS results show two populations with peak maxima about 150 nm and 380 nm. The Zeta potential was -3 mV (pH 7) across...

21 October 2020 6,551 1 View

What is the impact of the covid-19 pandemic on global warming?

Because of extensive lock up during the pandemic in many countries of the world, notably Europe, USA, Brazil, China and India, there was reduced use of hydrocarbons, especially due to much reduced...

15 October 2020 8,539 23 View

Is there any way to find RUL of bearing with incomplete dataset?

I am interested in finding RUL of bearing, which is currently in operation. I could find papers which uses run to failure datasets, which is not of my interest. Can anyone suggest methods or...

11 October 2020 7,962 7 View

Does anyone know of ontologies of the knowledge covered in US middle school and high school curricula?

For one of our projects we are looking for ontologies of middle and high school topics, such as math, science, history, and others. Is anyone aware of existing ontologies that are available for use?

10 October 2020 2,040 3 View

Multiple-Group Invariance with Categorical Outcomes AND correlated residuals?

Dear all, I have a question relating to the following paper: Dubravka Svetina, Leslie Rutkowski & David Rutkowski (2020) Multiple-Group Invariance with Categorical Outcomes Using Updated...

08 October 2020 3,142 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How to use measure capacitance change in COMSOL time dependent study ?

I want to measure change of capacitance due to relative displacement between two parallel plates in COMSOL. Distance between two plates changes with time. So I'm using a time dependent study but...

26 February 2021 8,567 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Missing Data in Pretest and Post test- Experimental Research design?

Hello all, I am analyzing some pretest and post-test data that were given to evaluate knowledge of targeted vocabulary before and after the intervention. Pretest and post-test are identical and...

23 February 2021 9,400 7 View