What is this species ?

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How to design a boost converter for a mppt controller?

I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...

28 February 2021 3,637 2 View

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Hello, I am using Coulomb 3.3. When I try to use the option "Build input from CMT or focal mech. data" I have the next errors: Unrecognized function or variable 'deg2utm'. Error in distance2...

18 January 2021 4,487 4 View

How do you prepare the 4X Laemli sample buffer for use?

Hi, I am preparing to do a western blot and I need to add 4X Laemli sample buffer to my samples before adding them to the gel and I had a few questions (I am using the Biorad 4X Laemli sample...

13 October 2020 9,485 1 View

Does anyone have experience with movement-based kernel density estimation (MKDE)?

Hello, I have mapped the locations of several individuals of a certain species of bird. I have the GPS coordinates, the date and the time data every half hour for several consecutive days and I am...

26 September 2020 3,712 2 View

A white mass inside the head of a cretaceous ant. What can it be?

I have a small piece of Burmese amber with a worker of the stem ant Gerontoformica sp. When focusing the head using incident light, it can be seen a white bilateral structure with several lateral...

26 August 2020 9,688 10 View

How can I generate TWDP random data?

Hi, I'm currently trying to understand the "Two wave with diffuse power" (TWDP) probability density distribution and test a method of moments estimator. However, I have a problem generating data...

20 August 2020 674 3 View

Do you think the teacher should switch roles with the students while teaching a foreign language through TIC?

There are some theories, as Pelgrum and Law (2003) suggested that it is necessary to make a switch between the teacher and the student roles. I mean, the student should be also the active role in...

15 July 2020 5,169 15 View

About Fortran and defining the units?

Hi to all. I am doing a large set of calculations employing as input files called 1.log, 2.log, 3.log,......, 300.log. Usually I use this definition for the input file: OPEN(5,FILE='...

12 July 2020 5,027 12 View

What is happening to my multiplex PCR?

I am developing a multiplex PCR assay to identify three strains of bacteria (A, B & C) of which two of them (A & B) are very close-related amongst each other. Two pairs of primers were...

27 May 2020 8,824 6 View

How do I detect primer dimers in an electrophoresis gel? and how to avoid them in a PCR reaction?

Hello! I am investigating plant-pollinator interactions by means of metabarcoding of pollen loads. The approach we want to use is dual-indexing in a two step PCR process. We are using four...

04 May 2020 7,455 6 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View

How to remove inter-year variation from my assessment of seasonal variation?

I have a 30 year dataset of fish sampling. I know from previous analyses that there is a huge amount of variation between years in catch rates. What I would like to do is create a plot that shows...

01 February 2021 3,980 5 View