I want to check the effect of plant pathogen on the integrity of host DNA.
Hi Jaisingh,
please try to detect nuclear fragmentation/degradation of DNA. It can occur in plants too, if programmed cell death is induced by pathogens. Look at:
http://en.wikipedia.org/wiki/DNA_laddering
Kind regards,
Maren
I have seen TUNEL assay reported very often for this purpose, but you could also try Comet assay or even PCR-based assays, depending on your goals. Here you can find some alternatives: http://www.excli.de/vol7/sinha02-08/kumari04-03proof.pdf
Thanks. Maren and Gustavo for suggestions....
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