I did a SNP array analysis on a leukemia sample. The SNP array karyotype showed chromosome 1q duplication with the size of 103.8 Mb. However, this duplication was not detected by the conventional cytogenetics. Does anybody have the explanation for this finding? Thank you in advance for your answer.
To my knowledge, SNP array karyotyping has more resolution than metaphase karyotyping, 103.8 Mb is too large to be a matter of resolution though.
May be you like to check this
http://www.bloodjournal.org/content/112/4/965/tab-figures-only?sso-checked=true
Hello,
have you consider that the cells with 1q duplication can not divide so easily, so you cannot detect them with conventional G-banding technique but only by array? Tereza
Tereza Jancuskova can you please explain the meaning of the cells cannot divide so easily? This is an interesting reason for my finding.
Yes of course, we have experienced that malignant cells sometimes did not divide in vitro and the result of convetional karyotyping was normal = 46,XX (or 46,XY). But with the use of FISH on interphase nuclei we have seen that the patient has abnormalities. So in this case malignant cells did not divide so we can not detect them via karyotyping. But I have to say that this was very rare and we experienced it two or three times.. .. but maybe it could explain your result.
May I ask you what sample it is (bone marrow or blood)? And diagnosis?
Tereza Jancuskova The sample is bone marrow. We extracted the genomic DNA from bone marrow. And the patient has childhood B-ALL, with normal karyotype by conventional karyotyping. There was no translocation. But the snp array detected 1q duplication with ERG deletion.
Sorry I have no reference cause as I wrote it was based on our personal experience and it is quite rare....
- Badges
- Science topic
- Similar topics
- Philosophy
- Philosophy Of Science
- Reasoning