What is the Principle of Division of Work?

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Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

Hello.. please how can I prepare an emulsion with nile red and nile blue dyes for visualization under fluorescent microscope ?

how can I incorporate the dyes to each phase ? do I have to heat them !!

21 June 2024 9,377 1 View

Is the Journal of Pedagogical Research is a trusted journal to publish in? I check in scopus and it is there but I have concerns if it is predator?

Is the Journal of Pedagogical Research a trusted journal to publish in? I checked in scopus website and scimago and I find it is there with scopus Q2 rank; but I have concerns if it is predator?...

08 June 2024 7,678 2 View

Topics for my PhD in TOPSIS?

I'm considering research topics for my PhD in TOPSIS methodology. Could you suggest some potential titles or areas of study that would be both relevant and impactful?

26 April 2024 602 2 View

Seeking Advice: K-Points and Band Configuration for Phonon Calculations?

I'm currently working on phonon calculations using Phonopy and VASP DFPT approach with a 2x2x1 supercell. I'm seeking guidance on: K-Points Selection: How should I determine the appropriate...

29 February 2024 3,955 0 View

Why may I get a Ct value that is higher for one of my treated samples than my control ( zero hour in a time course study) in RT qPCR??

I'm conducting a time course study on gene expression using RT-q PCR for samples treated with 4 conditions: vehicle, RA agonist, Calcitriol agonist or a combination of both agonists over 6 hours....

26 January 2024 7,921 1 View

How to dilute essential oil before antioxidant assay?

What are the solutes used to dilute essential oil for DPPH, TPC, FRAP and Beta/Carotene Bleaching Assay? Can I simply dilute the essential oil using 1 mg/mL in methanol/dH2O for all assay?...

21 November 2023 2,599 10 View

What style or the behavior of battery with draw compare with solar cell?

with draw the behavior of battery that meaning IV characteristic

09 November 2023 8,991 1 View

What is the possible reason why no emulsion formation in beta carotene/linoleic acid bleaching assay?

The procedure that I used 2 mg beta carotene in 10 mL chloroform with 200 mg Tween 40 and 20 mg linoleic acid. Next, chloroform removal by rotary evaporator and 50 mL of distilled water is added.

08 November 2023 1,890 2 View

What is the standard for Beta carotene/linoleic acid bleaching assay?

What is the standard/positive control that can be used instead of Butylated hydroxytoluene (BHT) or Butylated hudroanisole (BHA)? Sample: Plants’ essential oils

06 November 2023 2,250 8 View

In teaching with Evidence-Based Practices for students with ASD, what are some that can benefit from the use of AI?

I am a student in a master's program for Special Education and I am curious to see what Evidence-Based Practices that are used in teaching individuals with Autism Spectrum Disorder benefit from...

30 July 2024 5,611 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

I am looking for the permission to use the OLBI survey. Does anyone know where I can obtain that information?

I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...

29 July 2024 3,684 0 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

How to extract binding energy from pv.maegz file without using Schrodinger?

I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...

18 July 2024 2,881 4 View

Can a paper that is posted as a preprint in a database with DOI, can be reviewed in another journal and accepted for publication??

It happened that I am asked to review a paper for a journal. Incidentally, the paper is posted as a preprint in another database as open access with DOI. It is completely citable. Now, should I...

17 July 2024 1,831 4 View

What ethical dilemma is in predatory journals and difference between open source and closed source?

12 July 2024 7,514 2 View

Could someone please help me to understand the role of the quarter-wavelength principle in optimizing the thickness of perovskite solar cells?

How does the quarter wavelength affect the efficiency and light absorption of the cells, and what considerations need to be taken into account when applying this concept to optimize the thickness...

10 July 2024 647 5 View

What are the consequences of violating the code of ethics in research and consequences of breaching ethical principles?

10 July 2024 5,236 0 View

Authorship for data analysis?

I participated in 2 research projects in which I was responsible for part of data analysis and figures production. Also, I spent hours trying a new statistical approach -for me- to implement in...

10 July 2024 1,226 5 View