"Diagnosing mycobacterial infections quite often is a challenge. These organisms are very slow growers. They can be differentiated based on growth rate, niacin secretion, reduction of nitrate, caratogenesis, etc."
Specifically, some Mycobacteria contain the genes for production of the terpenoid pigments carotenoids. Mycobacteria are rich in the chemical precursors for carotenoids, and some species, like M.marinum, utilize carotenoids. Many environmental Mycobacterium have these genes.