27 November 2017 1 3K Report

My questions want to know how we can measure regulatory services of forest ecosystem and how these services impact on community resilience. I need to develop a methodology regarding this question.

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How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How to resolve ‘[ atomtypes ] Invalid order for directive atomtypes’ error in Gromacs?

I have been using Gromacs 2021.7. I wanted to simulate two small molecules in water. But, while generating the ions.tpr, I am getting the following error. The topol.top and .itp files for each...

17 July 2024 9,897 4 View

I am conducting an experiment for oxidative stress assay for C.elegans for a phytochemical and for FUDR needed to added at L1 or L4 stage?

and will i add phytochemical along with FUDR?

28 June 2024 6,254 0 View

How to provide minimum cluster size in lammps?

I want to calculate cluster sizes for a box of particles using the following method: # Compute cluster IDs for atoms compute myCluster all cluster/atom 0.9 # Define chunks based on cluster...

06 June 2024 5,273 2 View

How to analyze and quantify mitotracker confocal images?

I have been trying to study the mitochondrial morphology using mito tracker dye. But unfortunately I have not been able to find a way to analyze the images. Could anyone give any advice on any...

02 June 2024 6,183 0 View

In Design Expert optimization software, in ANOVA df values are not shown. What may be the reason?

I have analyzed my responses and valid p value was obtained but didnt show df value

26 May 2024 916 2 View

How to calculate the relative expression of a set of marker genes within various sorted populations from the same sample?

I have a WT sample from which we have sorted various cell populations via FACS. As a control population, we usually sort a pool of mixed cells from the same sample. Using RT-qPCR, I simply want to...

23 May 2024 6,992 1 View

How to get projections of spin-orbit entangled Jeff = 1/2 and Jeff = 3/2 states of 4d/5d compounds in VASP ?

In VASP orbital projected density of states (DOS) can be plotted without introducing spin orbit coupling (SOC). But how spin orbit entangled Jeff = 1/2 and Jeff =3/2 states can be plotted in DOS...

31 March 2024 5,934 0 View

Requesting coauthors for adventure tourism?

Geographical area: Southern bank of Yarlung Tsanpo, South-Tibet, Bumdelling-Tawang corridor, Eastern Bhutan. Keywords: Non comsumptive-Tourism, Soft Adventure, IBA, CCA, Host community,...

29 March 2024 3,757 0 View

Will there be a change in bandgap energy of AgNP exposed under blue, red and visible wavelengths of light?

Will there be a change in bandgap energy of AgNP exposed under blue, red and visible wavelengths of light?

22 March 2024 2,317 2 View

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