Need to know the Ideal range of susceptibility of Oxytetracycline in MHA media for Gram negative bacteria. I've searched literature but not finding the actual ideal range for Oxytetracycline.
The ideal susceptibility range for oxytetracycline in Mueller-Hinton Agar (MHA) media for Gram-negative bacteria can vary depending on the specific organism. However, the general breakpoints established by the Clinical and Laboratory Standards Institute (CLSI) are as follows:
- Susceptible (S) : ≤ 16 µg/mL
- Intermediate (I) : 32 µg/mL
- Resistant (R) : ≥ 64 µg/mL
These breakpoints can help you interpret the MIC (Minimum Inhibitory Concentration) when testing different Gram-negative bacteria. It’s important to refer to the specific guidelines and literature for the bacteria you are studying, as breakpoints may differ slightly between species. Always ensure that you are using the most current guidelines from authoritative sources like CLSI or the European Committee on Antimicrobial Susceptibility Testing (EUCAST).
The ideal range of susceptibility for oxytetracycline in Mueller-Hinton Agar (MHA) for Gram-negative bacteria is generally interpreted according to standardized guidelines like those from the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST).
As of recent guidelines:
1. **Susceptible (S)**: An inhibition zone diameter of **≥ 19 mm** is considered susceptible for most Gram-negative bacteria.
2. **Intermediate (I)**: An inhibition zone diameter between **15-18 mm** indicates intermediate susceptibility.
3. **Resistant (R)**: An inhibition zone diameter of **≤ 14 mm** indicates resistance to oxytetracycline.
These values may vary slightly depending on specific bacteria, and it’s important to consult the latest CLSI or EUCAST standards as they update these ranges regularly.
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