Is it possible that a protein band appears on a western blot but not detected on ELISA?

I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...

14 October 2020 5,144 4 View

How to calculate new human dose based on In-vivo animal study results?

I have performed In-vivo study in rate model for new formulation as well as marketed formulation. Based on results new formulation have significant improvement in all PK parameters. Oral dose...

20 August 2020 2,209 1 View

How many GCMs exist currently? Which are the most prominent ones among them?

General Circulation Models or Global Climate Models are over 40 when I went through a research article long back. I would like to know how many GCMs are currently in use. Names of them aren't what...

03 August 2020 2,789 5 View

Is it possible to fix a co-culture of bacteria and human cells in 96 well plates and use the plates at a later point for flow cytometry analysis?

I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...

09 July 2020 3,106 5 View

Is groundwater content considered in SM2RAIN algorithm?

I have come across SM2RAIN algorithm and can't help but wonder about groundwater content. I couldn't notice its inclusion in the algorithm. Is it something I've missed or I need to refer further?...

09 June 2020 5,272 6 View

Is there any MATLAB code for PHOTONIC CRYSTAL FIBRE SENSOR ?

I want to write code for a photonic crystal fiber sensor based on COMSOL multiphysics software ...can anyone provide me some idea regarding this ??

08 June 2020 3,100 1 View

How to do regridding using MATLAB?

I have a batch of netCDF files of precipitation data at 30km * 30km resolution. I attached a sample file of it. I want to convert them to 10km * 10km resolution using MATLAB. I tried using...

12 May 2020 6,007 6 View

Can someone suggest the most reliable database to check to understand the COVID 19 spread both globally and particularly in India?

I would like to map the spread of COVID 19 in specific locations to understand the social and economic parameters that could have caused the spread on the contagion. In this context, the data from...

30 April 2020 5,953 17 View

What are the uses of extreme values in Hydrological aspect?

For the research work I'm working on, I came across usage of extreme values while studying about deriving IDF curves for future climatic scenarios. What kind of results can I produce with extreme...

27 January 2020 4,145 4 View

What are the alternatives of Global Climate Models? Apart from downscaling, any methods exist to derive local climate variables from GCMs?

GCMS are used to predict atmosphere and climate behavior. Apart from GCMs, are there any methods to predict climate behavior? In GCMs particularly, are there any methods to derive local-scale...

02 January 2020 7,752 14 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View