Hi Greetings every one..! What is the full form of a fluorescent dye VIC that is used for labeling primers/ oligos? I googled a lot but could not get. It is a green coloured proprietary dye of ABI. I want to know the full form of the abbreviation VIC. I want to use it in my research paper.
This is the patent of the mention dye
https://patents.google.com/patent/US8962820
the full name is :
2′-chloro-7′phenyl-1,4-dichloro-6-carboxy-fluorescein
Gene Link does not offer Vic dye any longer and suggests Hex as a possible substitute.
VIC is a fluorescent dye that was originally developed by Applied Biosystems, but is now proprietary to Life Technologies. VIC has an absorbance maximum of 538 nm and an emission maximum of 554 nm, thus emitting in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5’-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications) and is currently only available from Life Technologies. Since the fluorescent dye HEX has a similar emission profile (absorbance maximum = 535 nm; emission maximum = 556 nm), for those customers who are able, Gene Link offers HEX as a reasonable substitute
VIC is a fluorescent dye that was originally developed by Applied Biosystems, but is now proprietary to Life Technologies. VIC has an absorbance maximum of 538 nm and an emission maximum of 554 nm, thus emitting in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5’-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications) and is currently only available from Life Technologies. Since the fluorescent dye HEX has a similar emission profile (absorbance maximum = 535 nm; emission maximum = 556 nm), for those customers who are able, Gene Link offers HEX as a reasonable substitute. For more information on HEX, please see its technical sheet.
Does it mean HEX can be used by telling the machine that VIC is used?
All the best,
In fluorescent dye name "VIC", an abbreviation "VIC" stands for "victoria". How and why? It has been developed after the modifications of Aequorea victoria Green Fluorescent Protein. I used this dye for getting primers fluorescent labelled during my doctoral work.
En effet VIC est encore appelé VICTORIA car developpé à partir d'une protéine Acquorea victoria
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