What are the QUALITATIVE INTERPRETATION OF Attitudes Towards Mathematics Inventory (ATMI) ?

Can you help me interpret the result of of the ATMI different factors..how can i interpret it as how low, moderate, or very high the result is of the factors.

29 March 2020 1,893 3 View

How to use the math attitude inventory?

how can i contact sir wayne w. Welch? I want to ask permission if i can use and guide how to use the Mathematics attitude inventory?

08 February 2020 6,084 3 View

How to image Methylene Blue stained Nylon Membrane?

I'm performing a Dot Blot and using Nylon Membrane stained with Methylene Blue for a loading control. Does anyone have any advice on how to image this membrane? I've tried using our Li-Cor Odyssey...

22 January 2020 2,178 2 View

Can ammonium sulphate acidify sterile media?

I recently did an experiment where I created sterile media by adding 1mM of ammonium sulphate to Murashige and Skoog (MS) Modified Basal Salt Mixture (No Nitrogen) M531. The media was not buffered...

30 October 2017 3,574 4 View

Hi all, Does anyone have experience coding custom non-linear regressions in Sigmaplot?

20 January 2015 4,874 1 View

Why is the supernatant from my pET expression system lysis step so viscous?

I have been expressing and lysing this construct for years, and today I got a thick gel instead of a nice supernatent. I recently started using Magic Media instead of IPTG induction, but this has...

05 September 2014 5,403 4 View

What types of transfection reagents do people use to transfect neurons, IPS derived neurons, astrocytes and neural stem cells?

Do more people use cDNA, siRNA, mRNA or miRNA? Would love to hear what people are using?

29 January 2014 1,147 7 View

What types of transfection reagents are you using to transfect neurons, astrocytes, IPS neurons?

cDNA, mRNA, siRNA, miRNA

27 January 2014 4,591 3 View

I have two pH meters with the same brand but with significantly different results. Why is this and how should I solve this problem?

I have two pH meters with the same brand but with significantly different results. Why is this and how should I solve this problem?

30 September 2012 9,441 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How to calculate the sample size required to detect the difference in pharmacokinetics of methotrexate?

I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...

14 February 2021 3,017 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to calculate ultraviolet dose in Gy or J/kg?

Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...

11 February 2021 7,642 3 View