Can someone help me with dosing ultra short and short peptides? Cytogenes and cytomaxes? thanks
Hi! I am graduating with my masters in Marriage & Family therapy the end of February & want to continue my education with a PhD. I applied to Florida State but that did not work out. What...
07 February 2024 3,341 2 View
We are slicing our human hippocampus with cryostat and wanted to know how much RNA (in ug) can you obtain from a cryostat slice? Obviously it depends on the thickness, but if anyone has any...
21 January 2024 715 2 View
We have a Jeol JSM 3690 SEM. The filament burned out and I replaced it and checked the objective aperture for damage. Cleaned everything well and reassembled. No amount of alignment, filament...
27 October 2023 297 7 View
looking for discussions and examples of cyber attacks on operational technology in any industry
02 September 2023 7,521 2 View
In the research design, MLRA was proposed since relationship is to be determined between several independent variables and a dependent variable. Nevertheless, the results was found to be not...
21 March 2021 1,083 5 View
A survey study in progress regarding completed testing methodologies for safety & security findings.
31 May 2020 6,806 0 View
help me identify the statements that fall in self confidence, enjoyment, motivation and value in the ATMI by Tapia, and help me also ask permission to use the ATMI as my research instrument....
21 April 2020 1,865 0 View
Can you help me interpret the result of of the ATMI different factors..how can i interpret it as how low, moderate, or very high the result is of the factors.
29 March 2020 2,062 3 View
how can i contact sir wayne w. Welch? I want to ask permission if i can use and guide how to use the Mathematics attitude inventory?
08 February 2020 6,315 3 View
I'm performing a Dot Blot and using Nylon Membrane stained with Methylene Blue for a loading control. Does anyone have any advice on how to image this membrane? I've tried using our Li-Cor Odyssey...
22 January 2020 2,318 2 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
we want to produce chocolate milk industrally .Dose anyone have a specific stabilizer formula for this pourpose?
10 July 2024 3,791 1 View