im looking forward for differences in any of the stages/proteins involved/others respectively
I think the principle should be the same.
I have done potentiodynamic polarization (Tafel) studies for a few surface modified samples. The intercepts and slopes were done exactly by the origin software. Its results show a gradual...
06 April 2023 1,226 1 View
I need to use it in the medical application.
03 February 2023 8,502 6 View
I need to determine the surface roughness of a curved or cylindrical item created using a 3D printer.
27 January 2023 4,235 4 View
I need to do novel tank test and light/dark test for zebrafish. So I need a tracking software for tracking in these tests. Is there any free softwares available for tracking of zebrafish ?
16 February 2021 1,890 1 View
Actually 316L Stainless Steel is the alloy. So how i can calculate for its equivalent weight?
14 May 2020 5,203 5 View
Dear Researchers, I am working on titanium alloy metal corrosion resistance. I am running EIS (10e5 to 10e-2) and Tafel plot. but my OCP is not stable even after 10hrs. I have done plasma arc...
06 December 2018 8,743 4 View
Dear Researcher, For biosheet designing blending Gelatin (G)- Alginate (A) and Chitosan (C)-Alginate(A) what cross-linking agent can be incorporated? Since I have also incorporate a nano particle...
11 September 2018 8,139 3 View
I am curious about the potential use of Ultrasonic stirring to to refine the grains and spread out the eutectics in High Pressure Die casting of aluminum alloys ?
20 May 2018 2,237 3 View
Lath Martensite: How many packets are in one prior austenite grain? How many blocks are in one Packet? How many laths are in one block (approximately)? what is the role of orientation relationship?
21 September 2017 2,004 4 View
Hello all, I am working on NIJ level 3A and 3. I have used UHMPE UD laminated fibers for the impact protection. My question, 1. Can I use same UHMPE UD laminated fabric for level 3 and 3A. 2....
23 July 2017 6,227 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View