Thanks for this question
You can add a drop of methylene blue solution to the sediment as mentioned by (Hansen and Perry, 1994).
“ Eggs of Fasciola species show yellowish colour while eggs of Paramphistomum species stain by methylene blue “
Reference:
Hansen J, Perry B (1994). The epidemiology, diagnosis and control of helminth parasites of ruminants. A hand book, International laboratory for research on Animal Disease. Nairobi, Kenya. pp. 31-36.
Thanks for this question
You can add a drop of methylene blue solution to the sediment as mentioned by (Hansen and Perry, 1994).
“ Eggs of Fasciola species show yellowish colour while eggs of Paramphistomum species stain by methylene blue “
Reference:
Hansen J, Perry B (1994). The epidemiology, diagnosis and control of helminth parasites of ruminants. A hand book, International laboratory for research on Animal Disease. Nairobi, Kenya. pp. 31-36.
colour, size and internal egg contents, using methylene blue stain.
I agree with Dr Al-Warid and Dr Sanchez.
Maybe the work by Bonita & Taira: Faecal examination of Fasciola eggs fixed with formalin solution using the beads technique. Veterinary Parasitology, 67(3–4), 269-273, 1996, can help you.
Regarding Paramphistomum, please be aware of the possibility of occurence of other Paramphistomidae genera such as Calicophoron, Cotylophorum.
Best regards
Hudson
Yes Ariff, the eggs of Paramphistomum are relatively larger in size
When both eggs is in a same microscopic's camp, there isn't problem, because the size and colours show big differences. The problem is when we are watchig only one egg of anything species. In this case, the Fasciola's eggs has a operculum on the blunt side, whereas the Paramphistomidae's eggs has a operculum on the sharp side.
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