What is the detailed protocol for APTES coating of glass slides? I am using using 2% APTES in acetone but there is heavy section loss.

09 September 2013 1 3K Report

I am performing cryosection of brain.

first you could include the brain in 4% agarose. adds the adhesive, and finally have the cuts. agarose gives extra support to the soft tissue.

Saludos desde Aguascalientes México. Suerte.

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