What are the best references for experiences on the implementation of simulation software in undergraduate teaching?

I am looking for benchmarks in educational studies that investigated the benefits, obstacles and disadvantages regarding the use of simulation software to support teaching at undergraduate level....

24 August 2020 6,968 9 View

Is this some type of contamination or just clustered dead cells?

I have few experience working with AN3CA cells. I seeded single cells in a 96 well plate and many of the wells have these structures and they are found with or without colonies of AN3CA that came...

09 May 2020 8,725 1 View

Any help with neutrophil IF in FFPE liver tissue?

Hi all, I've been trying to get an IF for neutrophils (using an anti-CD66b antibody) in FFPE liver tissue sections to work, but no success so far. The protocol is run on a Ventana automated...

04 June 2019 2,350 3 View

How to choose emg signal processing?

I have used two methods to process the same electromyography signal and calculate the maximum amplitude of it (RMS vs smoothing). However, I do not know which of them is the most appropriate,...

12 March 2019 9,524 4 View

Please, could you give a very short explanation of statistical methods used in species identific./reclassification by means of molecular markers?

This is a question on statistical methods used in DNA research Lately, many plant species are changing from species, genera, or even families due to studies in molecular makers. For example,...

07 September 2018 5,371 3 View

Is it crucial to use ATCC recomended medium to expand cell lines upon arrival?

03 April 2018 7,223 4 View

Reference gene for relative expression studies in mature sperm?

Which housekeeping gene could I use for relative expression analysis in mature rat sperm?

03 May 2017 2,944 3 View

Transform a closed questionnaire response from ordinal to intervalar variables

I have a closed questionnaire to access student's knowledge of a topic. Each questions have several (3, 4 or 5 generally) options. In each question there are one true answer and several not true....

08 April 2017 2,936 4 View

Antibody for IL-17 detection using IHC in mouse liver samples?

Hi everyone! Does anyone know of an antibody anti-IL-17 for IHC in mouse liver tissue (FFPE)? I'm trying to detect Th17 cells, but every paper I can find deals only with human cases. Thanks in...

31 January 2017 3,703 3 View

Microorganisms' Yield coefficients database?

Does anyone know if there is a database of known yield coefficients of microbial growth on different substrates?. Lets say for example, of the yield coefficient of yeast growth on glucose. Thanks...

04 October 2016 3,391 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

What program can I use to caclulate dipole moment efficiently and without the need of excessive computation?

Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...

24 February 2021 1,384 3 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

How do I refine a specific region of my protein model so that it perfectly superposes with a template peptide PDB with the same sequence?

I have a protein model and want to replace a 12-amino acids-long region within the center of the model with those of a PDB file containing a 12-amino acids long peptide of the same sequence. This...

02 February 2021 779 2 View

How to obtain the plot of frequency shift w.r.t. pressure in a micro resonator (cc beam) based MEMS pressure sensor in COMSOL?

I need to know how to plot the impact of pressure (0 - 100 kPa) (which is applied on the back side of diaphragm) on the frequency shift caused in the cc beam supported on the diaphragm with...

01 February 2021 5,805 17 View

Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?

26 January 2021 4,064 3 View

Where can I find the receptor binding domain (amino acid sequence) of ALL pathogenic viruses?

I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?

25 January 2021 3,843 4 View

How do I dissociate an antibody used for positive selection of cells in order to re-culture the selected cells?

Hi, I am interested in cells that express a specific receptor. I use a fluorophore-conjugated antibody bound to the receptor followed by MACS positive selection to enrich for this...

21 January 2021 2,245 3 View