What is the best way to sterilize/disinfect ivory slices for an osteoclast resorption assay?

More Katharina Kubatzky's questions See All

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How can I open a SXV4 file (SuperCROSS) in SPSS, R or STATA?

Hello everyone, I received some data from Statistics South Africa in an SXV4 format (SuperCROSS) and would like to open it in SPSS (or a similar programme if need be). I have tried opening it...

05 January 2021 2,463 4 View

Flow cytometric analysis of NBDG-uptake and intracellular staining possible?

Hello everyone, I am measuring glucose uptake into my cells by using NBDG and flow cytometry. Normally this works perfectly fine, but now I got some problems. When I fixed and permeabilised my...

25 October 2020 3,416 5 View

Does anyone know how I can see which nodes I chose for a specific file in NVIVO?

Hello! Does anyone know how I can see which nodes I chose for a specific file in NVIVO? I appreciate your answers, Katharina

02 October 2020 110 3 View

How can I analyse the modal ethnicity of a household and compare this to the ethnicity of the household head (SPSS)?

Hello everyone, I am working with a household survey and have both a person and a household file. I am using the household file for the main part of my analysis but want to explore the household...

21 July 2020 7,716 2 View

Who is working with Alzheimer's brain data?

Hi everyone, I am looking for a typical Alzheimer's brain for teaching purposes. I need a .nii file or similar. Of course the file should be showing a very typical brain and must be...

25 November 2019 7,917 3 View

Moderated mediation in PROCESS - Interaction not sig?

Hello, I'm computing moderated mediations using PROCESS for SPSS. The interaction effect for the moderation is not significant, however, the entire model for the moderated (!) mediation is...

16 December 2018 7,307 2 View

Did anybody experience crystal formation in BrdU ELISA (Roche)?

We use the BrdU ELISA Kit from Roche a lot & never had any problems with it. Somehow, now we observe crystal formation (dark violett to black crystals) in the last steps of the assay... we did...

26 March 2017 3,668 2 View

How do you connect individual short tracks in FIJI Image J Trackmate?

Hey there, has someone used FIJI Image J Tackmate before and could give me some advice on altering automated tracks? I'm trying to determine swimming speed of larvae. Trackmate detects the larvae...

28 February 2016 7,894 2 View

Who can help me with contingent valuation for a project?

I want to start a project for which I want to use contingent valuation. I thought about an online questionnaire asking people's WTP and WTA, but I have never conducted such a study. Is there...

04 March 2015 2,923 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Can anyone help me with doing resorption pit assay on biomaterials for 28 days?

I culture RAW 264.7 cells on my samples and differentiate them to osteoclasts with RANKL. I want to do resorption pit assay by culturing cells for 28 days. My problem is that the macrophages keep...

01 February 2021 6,746 2 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

How much of cells needed to get good number of RNA ?

Hello I am growing MSCs cells in either 24 wells or 12 wells plate and then I need to lysate these cells for RNA extraction I ususally get RNA from 5 to 30s ng/ul Is there is any idea or tips...

26 January 2021 8,531 3 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

Hello, my name is siti. My question is what is the related issue in bone marrow transplant or hematology that I can choose for my research project ?

I am doing a master in nursing but still does not have any idea about what topic I am going to choose. pls help

25 January 2021 1,097 12 View

Karan M Shah

We usually sonicate them for 2x10mins in distilled water and store them in 70% ethanol till we use them in culture. On the day of use, we wash them with PBS prior to cell seeding.

More details can be found in http://link.springer.com/protocol/10.1007%2F978-1-61779-367-7_23 published by our group.

Katharina Kubatzky

Haven't checked this section for a while, but thanks a lot for your answer and the link for the protocol !!!!