I need to prepare dry amylopectine powder from potatoe starch. Centrifugation seems to be the most appropriate way. Should I use any sedimentation agent to precipitate it conveniently?
Ethyl alcohol and centrifugation probably is the most convenient way. But could you give more specific details how do you obtain pure amylopectin?
It depend on how you purify and what do you want to do... Did you already purify the entire starch ? (if not you can use a percoll at 90% qnd centrifuge as explain in Deschamps et al. 2006). After you have to boil to separate amylose and amylopectin. Then the better methods to separate both is a size exclusion chromatography type Cl2b. The amylopectin extract could be dialyzed (if you use a salt in the Cl2B) and desicated.
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