What is the best mobile phase for isolation of compounds from Aloe vera flower extract?

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06 July 2019 6,878 3 View

Protein size decreases after mutation?

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04 May 2019 5,066 15 View

BL21 pLyss transformation and chloramphenicol?

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Why protien not eluted after changing buffer ?

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07 August 2018 4,340 4 View

How to make protien soluble during expression ?

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05 June 2018 8,574 6 View

Use of precukture ?

I want to ask why we always do a starter preculture step during protein expression ? Why don't we just start the main culture directly ?

04 May 2018 4,572 2 View

Protien not purified using ion exhange?

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Chemical shift of acetyl gp?

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11 December 2017 9,505 3 View

Methoxy gp on aromatic ring ?

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Isolation of soil microbes?

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08 September 2017 4,154 6 View

Is there a problem with my RNA pellet?

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How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

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The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

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Are air moisture harvesting technologies effective in combating desertification?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

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Low-yield gel extraction problem?

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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Manohar Padul

It depend on your targeted compound if your compound non polar you can use Hexane:Ethyl acetate

Gulab Dattarao Khedkar

If you are not sure about the chemical nature of the compounds you want to extract, you need to try different Mobile phase combinations. Try to use from non polar to polar solvents, and test for your requirement.