Dear Olcayto,

You can use the filament index. This method is very well described in the following book:

https://books.google.dz/books?id=wE8C7SVySbMC&pg=PA43&lpg=PA43&dq=microscopic+methods+for+determination+of+filament+organisms&source=bl&ots=3qICUK7Z1V&sig=HWM9XXuaIFsgm4VVZgnNvwyHy0s&hl=fr&sa=X&redir_esc=y#v=onepage&q=microscopic%20methods%20for%20determination%20of%20filament%20organisms&f=false   

With my best regards

Prof. Bachir ACHOUR

A quantitative method for measuring the mass concentration of the filamentous bacterium Type 021N in activated sludge using fluorescence in situ hybridization.

AIMS:This study aimed to develop a quantitative method for measuring mass concentrations of Type 021N, a bacterium causing bulking in activated sludge.

METHODS AND RESULTS:Fluorescence in situ hybridization was used to determine the relationship between the concentration ratio of the mass of the bacterium Type 021N to mass of activated sludge, and the proportion of fluorescence area imparted by probe G123T specific for Type 021N to that obtained with probe EUB338 for bacteria. A linear relationship existed between the cube root of the mass concentration ratio and square root of this area proportion.

CONCLUSIONS:A standard curve was obtained for quantifying Type 021N in activated sludge.

SIGNIFICANCE AND IMPACT OF THE STUDY:This method may allow the determination of growth rate constant of filamentous bacteria in activated sludge, information that will help in understanding their ecology.

Dear Prof Achour,

I have already been using FI method in our laboratory. However this book you offer me is newer than my Eikelboom's book. Thank you for your affinity.

Fluorescent in situ hybridization (FISH) method along with Eikelboom and Jenkins methods according to first link below.

http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6976.2009.00186.x/full

http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=0CDkQFjADahUKEwjs5cDTyNjIAhUI1h4KHQ6iBf4&url=http%3A%2F%2Fwww.epa.state.oh.us%2Fportals%2F35%2Fcompl_assist%2FTGlymph_Filament.ppt&usg=AFQjCNEVqK2GsiWMZUmXVk6c-DJKQPyKxw&bvm=bv.105841590,d.dmo

http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=0CFoQFjAIahUKEwjs5cDTyNjIAhUI1h4KHQ6iBf4&url=http%3A%2F%2Fwww.acsawater.com%2Fsites%2Fdefault%2Ffiles%2Fwebsitefiles%2FSVWWTPN%2FMicroscopic%2520Techniques.ppt&usg=AFQjCNHOjFUlPmNqb0YXtkbptZ0fFWPaZg&bvm=bv.105841590,d.dmo

http://www.ohiowea.org/docs/Activated_Sludge_Microbiology_Seman.pdf

http:/http://www.jesc.ac.cn/jesc_en/ch/reader/create_pdf.aspx?file_no=20000209&year_id=2000&quarter_id=2&falg=1/www.ncbi.nlm.nih.gov/pmc/articles/PMC383088/

http://www.acad.ro/sectii2002/proceedingsChemistry/doc2007-2/art02Aonofriesei.pdf

http://www.acad.ro/sectii2002/proceedingsChemistry/doc2008-12/art02Aonofriesei.pdf

http://homepages.wmich.edu/~maltby/PAPR3531/lectures/sludge-bulking.pdf

http://www.dec.ny.gov/docs/water_pdf/drrichard1.pdf

Hi Mr Thampi,

These sources may be useful for enumeration of filamentous organisms. I sould search for detailed information among them. Thank you very much for your helps.