What is the best management for this case?

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I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

How to do the tracking of deposition front ?

Hello there, i want to know what varibles should i use for the reinitialization parameter , as i want to track the solid deposition boundary with depletion of precursor vapors. how can i do it on...

03 April 2024 7,907 0 View

Can anyone guide or provide material to learn 2d analysis by nmr spectroscopy ?

I am beginner in the nmr spectroscopy can anyone help me how to interpret 2d analysis and structure elucidation. analysis type cosy HMQC HMBC NOESY NOE ROSY ETC

21 November 2022 4,329 2 View

In topspin software how to know how many sample run by analyst in one day can any one ideas???

How to know day by day number of sample run by analyst in topspin nmr software

06 October 2022 378 1 View

What are the input parameter values for steinberg guinan constitutive model used for lead core in ls dyna *MAT_STEINBERG?

Any one please provide the lead properties for steinberg - guinan constitutive model implemented in Ls - dyna as *MAT_STEINBERG.

03 October 2022 2,818 0 View

Is MAT162 mesh dependent?

I am simulating a FMJ bullet impact on a helmet, which uses MAT162 model for simulating its damage. when i tried for a coarse mesh(nearly 5mm element size) the bullet does not penetrated the...

03 October 2022 2,686 1 View

How can I separate two DNA fragments of 3000bp and 3700bp using polyacrylamide gel electrophoresis?

06 September 2022 9,723 4 View

What type of antifoam agents can be used for oil based fermentation process in bioreactor?

I am using vegetable oil as a carbon source for the bacteria and supplying air in the fermenter. During the course of fermentation, it is causing heavy foaming in reactor which is not getting...

06 July 2022 8,186 3 View

When plastein forms gel in plastein reaction when working protein hydrolysates?

I am working on protein hydrolysates of fish and performing plastein reaction using alcalase at 60 degrees for 6 hours. Can anyone suggest me the concentration of enzyme in percentage or about the...

26 May 2022 1,862 0 View

In acupuncture , has Bp been cured?

A patient had bp of ,170 by 13O and could hear his heart pounding. Pulse study Wood pulse was strong,and moving back vwards Understanding and findings Strong pulse indicates.excess Chi Going...

29 March 2022 4,000 1 View

Are you interested in contributing a book chapter on biomaterials in Orthopaedics?

I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...

29 July 2024 5,015 9 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

What is Printed Circuit Board (PCBs)?

Dear Members, We are conducting a research survey on the Printed Circuit Board (PCBs) industry and would greatly appreciate your valuable input. To participate, click the following...

09 July 2024 4,936 1 View

How does neuraxial anesthesia impact postoperative recovery compared to general anesthesia?

The Evolution and Application of Spinal Anesthesia: Techniqu... How does neuraxial anesthesia impact postoperative recovery compared to general anesthesia?

27 June 2024 4,159 1 View

Can you explain the differences between spinal and epidural anesthesia in terms of their mechanisms and clinical applications?

The Evolution and Application of Spinal Anesthesia: Techniqu... Can you explain the differences between spinal and epidural anesthesia in terms of their mechanisms and clinical applications?

27 June 2024 3,166 1 View

What are the primary indications for choosing neuraxial anesthesia over general anesthesia in surgical procedures?

The Evolution and Application of Spinal Anesthesia: Techniqu... What are the primary indications for choosing neuraxial anesthesia over general anesthesia in surgical procedures?

27 June 2024 5,763 1 View

What are the key contraindications to neuraxial anesthesia, and how are they managed?

The Evolution and Application of Spinal Anesthesia: Techniqu... What are the key contraindications to neuraxial anesthesia, and how are they managed?

27 June 2024 3,110 1 View

What are some of the most common complications associated with neuraxial anesthesia, and how are they prevented or managed?

The Evolution and Application of Spinal Anesthesia: Techniqu... What are some of the most common complications associated with neuraxial anesthesia, and how are they prevented or managed?

27 June 2024 8,659 1 View

How do you educate patients about the potential risks and benefits of neuraxial anesthesia, and what postoperative instructions do you provide them?

The Evolution and Application of Spinal Anesthesia: Techniqu... How do you educate patients about the potential risks and benefits of neuraxial anesthesia, and what postoperative instructions do...

27 June 2024 680 1 View

What preoperative assessments are crucial for determining a patient's suitability for neuraxial anesthesia?

The Evolution and Application of Spinal Anesthesia: Techniqu... What preoperative assessments are crucial for determining a patient's suitability for neuraxial anesthesia?

27 June 2024 3,525 1 View