Hello community,

I am measuring the distance between the Z-discs of in vitro derived cardiomyocytes. I usually fix them by 4%PFA after a brief wash with PBS. However Iam concerned that I can fix the cells when they are in somehow contracted state. I am thinnking about permeabilization and incubation without Ca2+, but am sure there is somebody with a protocol/experience to dispel my doubts?

Thank you very much for your reply

Vladimir

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