Hello community,
I am measuring the distance between the Z-discs of in vitro derived cardiomyocytes. I usually fix them by 4%PFA after a brief wash with PBS. However Iam concerned that I can fix the cells when they are in somehow contracted state. I am thinnking about permeabilization and incubation without Ca2+, but am sure there is somebody with a protocol/experience to dispel my doubts?
Thank you very much for your reply
Vladimir