How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Would someone be able to provide assistance with alizarin red s/mineralization assay with SaOS-2 cells?

I'm currently trying to induce and identify mineralization in my SaOS-2 cells. The issue I'm having is that the whole well will stain red, and of course this intensity depends on how long the...

26 January 2020 6,289 5 View

Does Mean Residence Time (MRT) and Elemental Ratio give better understanding about Biochar Carbon stability?

As MRT express the decay rate (average time that biochar can persist from decaying) and this rate decreases with time. Therefore, the incubation period becomes pivotal for the MRT calculation...

11 January 2020 6,697 3 View

Would someone be able to provide assistance with alizarin red s/mineralization assay with SaOS-2 cells?

I'm currently trying to induce and identify mineralization in my SaOS-2 cells. The issue I'm having is that the whole well will stain red, and of course this intensity depends on how long the...

31 December 2019 6,629 4 View

Is there any relationship between copper-uranium-gold-potasium porphyry, lead-zinc and hydrothermal regions?

We are researching on polymetallic magmatic-hydrothermal related to mineralization. Meaningful contributions and supporting journals shall be grately appreciated. Many thanks.

10 November 2019 7,390 5 View

How to quantify Alzarin Red staining of Bone FFPE slices?

Hi all, i am looking for a way to quantify the Alzarin red stain of my FFPE bone slices. My problem is that i want to compare the development of mineralization at specific time points and my...

10 November 2019 3,128 3 View

Spss Amos for new researchers?

I would like article or book recommandation for new researchers related to spss amos and SEM. Thank you

18 June 2019 5,764 6 View

Spss Amos for new researchers?

I would like article or book recommandation for new researchers related to spss amos and SEM. Thank you

05 June 2019 4,020 5 View

How to Improve the background in Amphiboles?

I'm working with Ca/Na amphiboles in granites and I'm looking foward to get some new insights about it. After the Book Amphiboles: Crystal Chemistry, Occurrence, and Health Issues from Hawthorne...

04 May 2019 8,974 4 View

What are properties such as young's modulus, poisson ratio, comppressive strength of asbestos ?

for usage as gasket purpose subjected to high temp and pressure.

01 February 2019 1,321 2 View

How to quantify mineralization of preosteoblasts on a hydroxyapatite scaffold?

I want to quantify the rate of mineralization of preosteoblasts ( MC3T3-E1) on a 3D scaffold. However, the scaffold is mainly composed of Hydroxyappatite (HA). Thus, I cannot use Alizarin Red...

11 December 2018 4,885 4 View