I am using PRP for Hair loss/Hair regrowth treatment. Currently I am using BD Vacutainer ACD Solution A 8.5Ml tubes.
I will explain the process that I am following currently.
I draw blood in 4 Vacutainer ACD Solution A 8.5ML tubes and centrifuge @ 3000 RPM for 10 minutes in 1st spin. Then I will transfer only buffy coat from the middle and little above buffy coat into another empty 15ml centrifuge tube and in 2nd I Spin it again @ 3500RPM for 5 minutes. Now I discard 75% of the above portion and remaining 25% I use for injecting into the Scalp and after injecting, I use 1mm derma roller on the scalp and then I just spread the PRP on the scalp.
Please let me know if there a better way to get the maximum PRP for hair loss treatment or if there is any change in the centification process.
What is the concentration of platelets in the final solution?
Which Activator do you use?
I am using Vacutainer ACD Solution A 8.5ML tubes. it contains ACD (Acid Citrate Dextrose) Solution A.
ACD Solution A contains 38mM citric acid, 107mM sodium citrate and 136mM dextrose
Solution A (per 1000 mL)
Total Citrate (as citric acid, anhydrous (C6H8O7)) 20.59 to 22.75g
Dextrose (C6H12O6*H2O) 23.28g to 25.73g
Sodium (Na) 4.90g to 5.42g
there is a study done several years ago and automatic Harvest Technologies (HT) centrifuge and Angel System got the highest platelet recovery: around 80-82%. Also the automated systems have shown better PRP quality and less contamination vs semiautomatic and manual centrifuges. HT uses Thrombin for platelet activation and release the growth factors.
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