I'm investigating the antidiabetic activity of a certain medicinal plant. In this plant, no studies have done fractionation and isolation of bioactive compounds. But, its related species contains flavonoids, saponins, terpenoids, tannins and others.
Dear Kelifa,
Your question is little confusing. Since your plant of interest is having flavonoids, saponins, terpenoids, tannins I suggest you to go for hydroalcoholic extraction initially. Further, you screen the activity in the cell line (Glucose uptake ability). If you find the promising results go for toxicity study followed by activity evaluation.
One you have confirmed the anti-diabetic activity, go for compound isolation by chromatography techniques (HPLC, LC-MS and NMR).
Dear John Kelifa,
Either you had been get a result as antidiabetic activity, It will be interesting or not yet investigated, your study containing two parts. You should first test aquas crud extract, with or without chlorophyll, to confirm present of active ingredients to aplly in your desired field. Otherwise, for the natural product extraction, first remove the chlorophyll and then perform TLC to select the best system of polarity for your extraction. Further, after you will continue in steps of extraction using chromatography techniques.
Dear Kandimalla,
Thank you very much for your kind advice. The dried plant material (root) has already extracted by 80 % methanol. In very near future I'm going to do acute oral toxicity study. Then I will go for in vivo activity evaluation. what are the solvents most likely I have to use for fractionation?
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