I have studies about cereal cyst nematode.
How to inoculate CCN nematodes in pots?
Is before planting or after ?
Dear Tayeb
J2s of CCN can hatch without extractions of host and in pots without host plant you may lost your populations of CCN. I think it is better that you inoculate after planting.
best regards
The best way you should inoculate with J2. it means you have to incubate the cysts (which species?) if you are working with avenae species it is more diff than filipjevi or latipons.
i can send you more info if you still need the such.
regards
First: do not inoculate cysts. You do not really know your initial population density when using cysts. Do not hatch them either. Try to crush the cysts, retrieve the eggs, make a suspension with a known population density, which you can inoculate with a syringe into the soil. Look at how it is done for PCN. The whole methodology is already developed.
Inoculate the eggs before planting. This is the real life: the nematodes are already there when you plant or sow your host. When you first let the plant grow, both population dynamics and yield losses are different from what is happening in the field. Read Plant Growth and Population Dynamics by Schomaker. Plants with an already established root system when attacked by nematodes, provide less space for females to rep[roduce and have a lower yield loss (same as using a granular nematicide)
if you don't hatch the cyst this will take months for some species to hatch which it means the trials will be terminated before the J2 appear. we are working on the CCN in wheat since years and we follow this as a standard methods in collaboration with many collaborators from around the world. i wish egg inoculation could help so we can eliminate the struggle of hatching.
thanks and regards,
First of all you should take initial population of soil or you take sterilized soil then you will grow seedlings and after that you will inoculate J2.
Though it depends on the type of crop you are going to inoculate you better inoculate after planting the crop. However, be sure that you determine the Pi in the soil you used to fill the pots. If you used steralized soil the you just need to inoculate < 24hr old J2s. You need to use root exudates to let the cysts hatch easily. You may use tomato or potato plants to prepare the root exudates.
I hope this piece will help you!
Awol
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