Problems with library virus generation?

Dear all I cloned an sgRNA library into a pLenti plasmid backbone (puromycin selection marker) by golden gate ligation and transformation. However I cannot produce any virus from the library...

25 October 2020 3,820 2 View

How can I create a Venn diagram using SPSS?

I have 5 sets with 200 variables.

14 October 2020 9,681 4 View

Transfection of HEK293T cells with linear dsDNA?

Does anyone have experience transfecting or electroporating linear dsDNA into HEK cells? If yes what is the size limit, what size have you tried and worked. In principle, I want to do HDR, and...

25 March 2020 5,649 3 View

How the Aluminium Oxide layer on Battery Terminals affect the performance?

Hello Everyone, I am currently optimizing the production process for the battery packs. I would like to know how the great the increase in contact resistance with an aluminium oxide layer on the...

21 May 2019 2,782 3 View

Why DCIR value changes in the Battery?

I am using LiFePO4 battery, I measured DCIR using the discharge pulse. I found that the measured value of DCIR changes with respect to both pulse current and SOC. I provided adequate rest period...

28 January 2019 5,227 4 View

How to measure the energy consumption by DC system?

I am designing Battery pack for a DC system. In order to find out the capacity required for the system, I need to calculate the energy requirement (Variable load, Idle condition). Since the...

10 September 2018 9,766 8 View

How to test BMS?

I would like to know how a BMS from a different Manufacturer can be tested? I want to make sure whether the BMS works as per our requirement and also ensure its operation as per the data...

08 May 2018 5,204 3 View

How to find out diffusion coefficient using warburg impedance?

11 September 2017 1,196 13 View

How to infer the data of Oscillation Amplitude Sweep?

04 September 2017 4,820 3 View

How to differentiate RC element from Warburg element in DRT?

In Distribution of Relaxation Time, how to differentiate whether the peak corresponds to a RC element or Warburg element?

06 September 2016 8,285 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View