I am going to make plasmid construction, contain certain scFv (small protein fragment approximately 30-100 kD) gene and express it by means of a common expression E.coli strain.
then it will be conjugated on Gold nano-particle surface for targeting purpose.
what factors must be considered in nano particles-protein conjugation???
Usually disulfide bonds are used for conjugation of molecule to gold nano-particle. if your protein has a cysteine, it is possible.
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First thanks for response .
It is possible to insert cysteine at the end of scFv (N-terminal).
One aa is enough?
Because your molecule is small, adding an amino acid may be enough. But in general, if the number of disulfide bonds is higher, you have a better conjugation of molecule to gold nano-particle.
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