I am trying to CHECK RNA quality using gel electrophoresis 1% gel and 6ul sample and 3ul 1x dye but the bands appeared smeared like that Who could be the cause? I used the Kit for extraction and the nano drop showed a high concentration of RNA.
Michael J. Benedik
Is this entire cellular RNA? First I am surprised you aren't seeing the rRNA bands but if you have enriched only for mRNA that might explain it. Secondly, I would expect total RNA to be a smear since the mRNA is of all different sizes.
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Amy Klocko
Sometimes the bands can be improved by briefly heating and cooling your samples just prior to loading (10 min or so, 65 degrees, then cool on ice). Only do this for what you are loading on the gel, not your entire RNA prep.
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