How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to use the results of Co-IP for protein quantitative difference calculation?

I did a Co-IP MS with two samples and got the LFQ data from MaxQuant, and I wanted to normalize it by an internal reference protein. After I divide the LFQ of the other protein by the LFQ of the...

18 January 2021 5,419 4 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

How to Draw Crystal Structure from powder XRD?

Hi, I would appreciate if anyone can recommend on the process of drawing crystal structure from observed powder xrd diffractogram and if there is any suggested software to work on.

05 January 2021 3,517 3 View

Why mass flowrates of atoms at the inlet and outlet of a reactor are not conserved (CFD with chemical reactions, species model - eddy dissipation)?

Hi, guys. Thanks for reading this question. I am modeling a reactor (steady, RANS). CH3OH and O2 go into the reactor via inlet (a jet). After reactions, species leave the computational domain...

08 December 2020 7,362 13 View

Is the pH value will affect the calculated overpotential in OER process by DFT?

27 November 2020 1,456 2 View

How to transform this implicit FDTD iteration equation into the matrix equation?

11 October 2020 9,042 1 View

What is the best way to evaporate a large volume of aqueous solution quickly?

Let's say I have 100 mL of an aqueous solution and I wish to reduce the volume to 5 - 10 mL. What's my best option? - Rotavap is ruled out because we do not have a vacuum pump that can create...

09 September 2020 2,534 8 View

Bugs in mouse genotyping?

Hi, I am genotyping newly constructed trangenic mice knocking down target gene. I have got F1 generation from F0. And DNA examination indicates that the target DNA fragment has been excised. The...

27 April 2020 5,450 3 View

Will there be a large temperature gradient in the cross-section of a steel member under fire?

I have been working on the behaviors of steel structures at evaluated temperatures. We all know that heat can be easily transferred through steel. So, in real cases, will there be a large...

19 March 2020 1,748 5 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

What are the available keys and descriptions for the identification of lice and fleas in domesticated animals?

I am an undergraduate student at the University of Peradeniya Sri Lanka, currently working on ecto-parasites in domesticated animals. I found difficulties in the identification due to a lack of a...

15 February 2021 277 1 View