Dear Ngozika, my co-worker Dr. Celenk Molva is expert for isolation and charcterization of Bt. You can contact with her.

https://www.researchgate.net/profile/Celenk_Molva

Celenk described procedures in paper entitled "Isolation and characterization of Bacillus thuringiensis strains from olive-related habitats in Turkey" which appeared in  Journal of Applied Microbiology 104 (2008) 515–525.

Best regards.

You can use primers for cry gene identification as bacillus cereus and bacillus thuringiensis have no genetic difference only crystal protein staining and cry gen amplification will help to identify.  

Dear Ngozika

please see attach file  i hope its help you

Best Regard

Dear Ngozika, my co-workerDr. Gholamreza Salehi Jouzani is expert for isolation and charcterization of Bt. You can contact with his. He has several paper about different aspect of Bt. His e mail is: [email protected]

Best Regards

 Dear Sir,

Mohammed Fayyadh

sir an error occur while downloading the attachment.

Dear  siddique

 i trying to download the file i hope you can download it.

Best regard

139

Hi Ngozika

Many useful references in attachment 1

https://www.omicsonline.org/open-access/industrial-production-of-bacillus-thuringiensis-based-bioinsecticide-whichway-forward-2155-6202-1000e126.php?aid=45240

2

https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100006

3

https://onlinelibrary.wiley.com/doi/10.1111/lam.12089/pdf

4

https://www.komunich.de/vincent-sanchis/article31.pdf

5

https://www.tandfonline.com/doi/abs/10.1080/03650340802158201

6

https://www.ncbi.nlm.nih.gov/pubmed/23594143

7

https://www.ncbi.nlm.nih.gov/pubmed/19149720

8

https://miami-dade.ifas.ufl.edu/pdfs/fyn/bt.pdf

9

Article Optimization of a fermentation process for bioinsecticide pr...

10

https://www.eolss.net/sample-chapters/c17/e6-58-05-08.pdf

11

https://eap.mcgill.ca/MagRack/JPR/JPR_05.htm

12

https://www.hindawi.com/journals/tswj/2014/273030/

13

https://sitn.hms.harvard.edu/flash/2015/insecticidal-plants/

14

https://www.jmb.or.kr/submission/Journal/017/JMB017-04-01.pdf

15

https://www.jbiopest.com/users/LW8/efiles/Vol_5_2_144-147-12013.pdf

16

https://entomology.ca.uky.edu/ef130

17

https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.334.635&rep=rep1...pdf

18

https://www.aces.uiuc.edu/vista/html_pubs/biotech/insect.htm

19

https://www.eid-med.org/download-form/dl-file/e951ccd95572a67138f4572c1c7d7ee8

20

https://file.scirp.org/pdf/NS_2013110413390145.pdf

21

https://www.bpia.org/history-of-biopesticides/

22

https://link.springer.com/article/10.1051/agro:2007054

23

https://biopesticide.ucr.edu/abstracts/assets/all_abstracts.pdf

24

https://isaaa.org/resources/publications/pocketk/6/default.asp

25

https://www.sciencedirect.com/science/article/pii/S135951130600359X

26

https://www.entomology.wisc.edu/mbcn/fea207.html

27

http://www.who.int/water_sanitation_health/gdwqrevision/RevisedFourthEditionBacillusthuringiensis_Bti_July272009_2.pdf

28

http://cdn.intechopen.com/pdfs/41358/InTech-Toxicology_of_the_bioinsecticides_used_in_agricultural_food_production.pdf

29

https://www.academia.edu/2893591/Development_of_a_Cost_Effective_Medium_for_Production_of_Bacillus_Thuringiensis_Bioinsecticide_Using_Food_Barley

30

https://archive.bio.ed.ac.uk/jdeacon/microbes/bt.htm