Can anybody clarify the importance and use of these values? Also I am having difficulty understanding the concepts of completeness, peptide occupancy, R-merge and Rcryst (Rfree) (%). Any explanations would be appreciated.
There is an enzyme which pH optima is 6.5, we want to make this enzyme working well at pH 4.5, without disturbing its catalytic efficiency for a reason of industrial fermentation process. The...
06 July 2018 8,991 6 View
I am getting Km value of a mutant which is similar to its wild type. How it is possible ? The residue is quite important for substrate binding. Is it possible ? please suggest !
10 November 2017 5,555 4 View
I am planning to make the wild type enzyme pH tolerant (acidic) without affecting the activity of the native enzyme. Here i am thinking to introduce some residues like lysine or ariginine in place...
08 September 2017 1,388 5 View
I am trying to find some tool or server which put secondary structure elements on top of the sequence alignment which ESPRIPT does very well. But is there any other tool which I can use to show...
02 March 2015 787 5 View
I have done whole plasmid amplication using XT-20 high fidelity enzyme from merck. After induction with 0.3mM IPTG i did not see any expression of the protein.I am suspecting some mutation has...
01 February 2015 3,166 6 View
Please suggest any protocol which is cost effective and feasible. Thanks in Advance.
09 October 2014 6,861 5 View
I am little bothered that my structure refined at 3.2 Angstrom, have a difference of 10 % without and with solvent contents. The difference is not changing. completeness is 97.6 % at 3.2 Angstrom....
06 July 2014 6,907 8 View
I have used a boxshade server, but here I am finding difficulties as it puts sequence numbers here and there after the job. So does anybody have an idea to do the same without any erroneous job of...
06 July 2014 9,161 4 View
I am creating a file for the ligand by generating the omit map in order to visualize it into pymol. Does anyone have any explanation what the Refmac sigma-AA weighted map data is, and why it is...
06 July 2014 7,204 5 View
As I have purified my protein and set crystallization screen and I tried various way to optimize the crystal quality, such as seeding, changing pH, PEG concentration, salt concentration and...
04 May 2014 8,624 10 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...
11 July 2024 1,801 2 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...
04 July 2024 8,100 4 View
Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.
23 June 2024 2,287 7 View
It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?
15 June 2024 1,355 7 View
Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...
13 June 2024 9,936 5 View
I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...
31 May 2024 6,444 1 View
What is the difference between absorption and adsorption?
30 May 2024 4,447 27 View
I'm interested in knowing the differences in the effects caused by four different types of biochar: cantaloupe leaves/stems, onion leaves/stems, grape peels/stems/leaves and asparragus...
28 May 2024 4,907 0 View