Would you please recommend a function in R software that could be used with FUNGuild output to merge different rows that have similar guilds?

Dear colleagues, I have an output of FUNGuild tool (ASVs abundance in different samples and assigned to guilds) and would like to merge the ASVs that had similar ecological guilds using R...

16 February 2021 4,292 7 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Why is the OD values not decreasing using 3 dilution in Indirect ELISA?

I am using indirect ELISA to measure the antibody response against Aeromonas salmonicida in Atlantic salmon. I am using 3 dilutions of serum 1:50, 1:200, and 1:400. The OD450 nm values measured at...

08 February 2021 9,049 3 View

What is the best etchant for severely deformed, high purity magnesium?

I have tried using acetic picral and 5% nital solution when observing the microstructure of severely deformed pure magnesium. Unfortunately, I keep getting similar microstructures (as shown below)...

20 January 2021 8,783 4 View

Can you recommend me chemical engineering journals with fees "scopes" ?

dear all would you help me to find a scops journal in the field of chemical engineering with fees as I want to publish in a short time. thank you [email protected]

23 December 2020 6,883 1 View

Sensitivity Coefficient in Uncertainty Calculations for HPLC assay method, UV assay methods and pH measurement?

Does anyone have a paper or any document for calculated sensitivity coefficients used in uncertainty calculations for HPLC assay methods, UV assay methods and pH measurement methods?? I know it...

21 December 2020 5,196 2 View

How to interpret PCA results in weka & how to extract features from it?

I'm working on KDD CUP'99 dateset. Training and test set are different datasets. after pre-processing I got 25 attributes. I applied 4 different classifiers through PCA in WEKA(classify tab). Now,...

20 December 2020 8,425 4 View

How can we define columns in 3D PLAXIS model for buildings existing near the excavation?

I started by a model using 3D PLAXIS to study the effect of deep excavations on existing buildings. The building was simulated as a surface plate with a surface load. Now I'm trying to do it as a...

29 November 2020 3,198 4 View

Add text at the beginning of a specific reference in Latex?

Hey researchers! The journal I'm submitting to wants datasets to be listed in the reference as [dataset] Authors; Year; Dataset title; Data repository ; Version; Persistent identifier (e.g....

18 November 2020 2,681 5 View

What criterion measures do you suggest to be used for a new children personality test?

I am working in a project that aims to develop a new non-verbal personality test for children. What king of measures/ intruments I can use to examine the convergent or divergent (or concurrent...

11 October 2020 1,960 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View