I want to make my gene construct as soluble devoid of signal peptide and helical amphipathy to avoid membrane anchor. Can anyone suggest few examples.
Thanks.
Try TMHMM (hydrophobic helix) and SignalP at the CBS :
http://www.cbs.dtu.dk/services/
It provides you accurate curves and suggestions to make a rational design with your sequences in fasta format, easy to use.
Thanks Jean
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