In most studies, microbiologists use electron microscopy (TEM or SEM) or confocal laser scanning microscopy. But are there other options, like determining a parameter in lung homogenates like proteins, genes?
Biofilm formation can be defined by attachment of bacterial cells to a surface combined with intercellular adhesion between bacterial cells resulting in large cell clusters. There are many factors involved in primary attachment and cell accumulation. Most often a single substance can not necessarely used as a marker for biofilm formation.
Also gene expression is not confirmative for biofilm formation. There are typical expression patterns in bacteria grown in a biofilm. However, these patterns can often also be presented in cell populations growing in high cell density without forming a biofilm.
Is it possible to use a species specific 16SrRNA Probe for visualizing bacterial cluster in biofilm associated with tissue surface.
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