http://www.biolegend.com/essential_markers

Thanks. Out of these NKT cells what minimum marker(s) would help identify the activated NKT s?

Hi Anjali,

For studying invariant NKT cells, it is best to use alphaGalCer-loaded or PBS57-loaded CD1d tetramers for detection (available upon request from the NIH tetramer core facility, I believe), in conjugation with CD3, CD4, CD8, CD56 stains, and some B-cell (CD20)and monocyte (CD14) exclusion markers.

For activated iNKT cells, I would recommend CD69.

All the best,

Pierre

Thank you so much for your tips.

Anjali.

Hi Anjali,

In addition to what Pierre said above, a novel monoclonal antibody (clone 6B11) is now widely used to phenotypically characterize human invariant NKT cells (Montoya et al., Immunology, 2007). 

Good luck,

Sudhanshu

Thanks for helping me.