Hello all, What are the different sources of variables in data being collected from siblings mice came from the same cage, given same diet, water, bedding, and handling?
The answer depends on the trait that we concerned, in the case of a qualitative trait the variation depends on genetic variance only and no environment variance. But in quantitative traits, the phenotypic variance consist of: genetic variance + environment variance+ G×E interaction variance.
The possible explanations coulde be individual biological variations, genetic variations, microenviromental variations and interaction of all these variations. Even in monozygotic twins, individual biological and microenvironmental variations play a major role given the fact of genetical identity of siblings. Further data measurement errors also should taken in to an account.
Rana, that variation could also have come from you too (we do not know much about the study design), there could have been some underlining factors (confounding) that may have introduced bias or variation. Also, just ordinary differences in birth weight of the sibling mice could be a factor. Also, If you give them same feed and same water, they may not be feeding at the same rate and at the same quantity.
Well, the mice are siblings, but did they come from a specific lineage? If not, there could be genetic variation among the siblings. Also, are all of the mice the same sex? I'm not certain what data you're collecting, but there might be some variations due to sex as well.