Hi Thomas, I have just started looking for the same references and I have found good reviews (https://doi.org/10.1016/j.eswa.2012.01.073 and Article Correction: Leaf Morphology, Taxonomy and Geometric Morphome...
) and tutorial (https://bio-protocol.org/e2269), and I have started using ImageJ. I think methods and analyses will depend on what is your question.
I dont know if it helps, but I have written some papers where I have used morphometric analysis of fish and described the methods and statistical analysis.
📷SourceReproduction and sexual dimorphism of daubed shanny (Teleostei: Leptoclinus maculatus) in Svalbard waters
Early life history of the daubed shanny (Teleostei: Leptoclinus maculatus) in Svalbard waters
Taxonomy, morphology and biology of Triglops murrayi and Triglops nybelini (family Cottidae) obtained at Svalbard and Jan Mayen
Hi Thomas, I have just started looking for the same references and I have found good reviews (https://doi.org/10.1016/j.eswa.2012.01.073 and Article Correction: Leaf Morphology, Taxonomy and Geometric Morphome...
) and tutorial (https://bio-protocol.org/e2269), and I have started using ImageJ. I think methods and analyses will depend on what is your question.
I think there is a rather large literature on this topic. A quick google scholar search with "morphometric analysis leaves" will bring up many hundreds of papers related to this type of question.
One of the first stops on the net might be at this site: http://life.bio.sunysb.edu/morph/
Thais Elias Almeida and Timothy A Mousseau , thank you for this information.
I am well aware of general concepts in morphometric analysis- however, what I am looking for is a more detailed methods paper. For example, if I am carrying out a taxonomic study, how exactly do I decide what leaf to measure on each voucher when there may be >30 leaves per specimen? Or maybe which node to examine for the presence of pubescence? Which petiole do I measure the length of?
Are these just selected at random?
The reason I ask is that most taxonomic studies/ methods papers just mention that they collected these measurements but never actually explain exactly how they were collected or what rules they assigned for selection of a leaf, node, petiole, etc.
Good points! You will have to review the literature dealing with these specific traits and determine if anyone gives some rational for their choices. Usually one will choose a character for which a homologous landmark can be found in all specimens, at a minimum. In addition, there may be some physiological, genetic or developmental reason for selecting a particularly point. It sounds like you may have some ideas along these lines.
I think it depends on the kind of species you want to study, for scaly-like leaves juniper I highly recommend this one :Article Taxonomic and geographic differentiation of Juniperus phoeni...