What are different theoretical approaches in archeological explanation covered in the introduction to archeology?

More Justin pindit Lasak's questions See All

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

Will rat colon cancer cells (DHD/K12) derived from BDIX rats grow in Sprague Dawley ?

we plan to induce colon cancer by injection. Cell lines derive from BDIX rats, but our experimental setting would favour SD rats. any experience?

23 January 2021 1,437 2 View

I am interested in sequencing small RNAs in bacteria but enriching for small, weakly expressed transcripts seems to be a challenge. Any ideas?

Assays for bacterial small RNA sequencing

06 January 2021 4,549 2 View

What is the white layer on my fermented natto bean paste?

Dear all, Good day! Recently i tried to ferment my blended soya bean powder mixed with Natto bacteria culture in a Natto maker (containing some water) at around 45 degree celsius overnight....

23 November 2020 7,105 20 View

What is the scenario for the gold price?

What do you think about the further growth of gold price? Do you think that the upward trend is coming to an end? Or may be we observe a temporary turbulences caused by the US presidential...

23 September 2020 1,344 4 View

How do I extract mice brains for multiple experiments?

I am looking to complete western blot experiments and IHC experiments on the same cohort of mice. Is it possible to Harvest the mouse and use the right hemisphere for IHC and the left for western...

08 September 2020 5,436 3 View

Why the need to have CIE formula when color difference can be measured with RGB values?

Dear all, Recently i have been trying to measure colour difference between my samples. I am a little confused as to why i cannot just use the basic colours - i.e., red, green and blue to measure...

02 August 2020 7,375 2 View

Does anyone know of an Antibody against against Mouse Microglia trf2 that is good for Immunofluorescence and Westernblot?

Hi, I am studying the knockdown of Trf2 protein in Murine microglia cells invitro and wish to demonstrate this knockdown via immunofluorescence and Western blot. A dependable Trf2 antibody and...

02 June 2020 8,682 3 View

Should I use log transformed data for correction then revert it back?

Hi, I'm trying to merge two chlorophyll datasets from different sensors by applying linear regression on their data overlap. The journal that I'm following log transformed (base 10) the data from...

02 April 2020 871 7 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is the logarithmic transformation a necessary step in time series analysis ?

Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...

15 February 2021 5,655 14 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Mixed ANOVA with some normal and some non-normal data?

I am looking to run a 2x5 Mixed ANOVA to examine Sensory Processing Sensitivity in Highly Sensitive People (HSP) and Non_HSP. My Between-Subjects factor is HSP and Non-HSP. All participants tested...

11 February 2021 1,445 6 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Age of the Isimila Prehistoric Site in Tanzania?

Does anyone knows results from the new dating program from the Isimila Prehistoric Site in Tanzania?- of course without to give away too much, before a final publication. The data of ca 260...

03 February 2021 6,864 3 View

After transformation of Dh5alpha competent cells I found a cloudy white ppt before plating. Can anyone explain this?

After transformation of Dh5alpha cells by heat shock I grew them at 37 in 200microlitre Terrific Broth (because I had it at hand) and then before plating I found cloudy white ppt in the cell...

03 February 2021 7,867 3 View

Simon Lysaught

Looking at the exam leads me to believe this question is only answerable with knowledge of what exactly is discussed within the course in question.

Penina Emanuel

Cognitive archaeology

Ricardo Ojeda García

Probably option C, those are what we would call "archaeological theories" that set the floor to explain what we find in the archeological record by integrating them into a set of principles that explain the evolution and behavior of human societies.