I am simulating a predator-prey interaction between the species Myxococcus xanthus and Caenorhabditis elegans, with the bacteria being the prey and nematodes as the predator.
(A brief) Experimental setup is as follows:
-Plate M. xanthus onto standard 1.5% CTT media-let grow in isolation at 32C for 48 hrs.
-Bleach synchronize populations of C. elegans, wash for 48 hrs. Plate onto M. xanthus.
- Allow interaction to occur over 5 days
After 5 days, odd crystallization formations began appearing on my plates. I concluded that this is occurring due to the interaction, as my plates with only M. xanthus had no sign of these formations. I am unsure of what they are, and what is causing these to appear. I have not found much information in the literature. Has anyone seen these before? Attachments are included.
This is extremely interesting! Cool photo's!
I don't know anything about any of the 2 organisms, but do the nematodes stay alive in the cultures that make the crystal structures? Did the bacteria from endospores in the culture? Are the plates still kept at 32C after the nematodes are added?
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