Both I and another investigator have performed this assay and our experimental values are about 5 fold lower than expected. I also get a 5 fold lower result when glycerol is used instead of serum. I perform a 5-point standard curve with each assay and it is very similar each time. I have searched Wako's website and e-mailed tech support (with no response) trying to find typical values for the standard curve but could not find anything.
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