What about NPs extraction?

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What is the best statistical method to analyze temperature effect on insect biodynamic?

Hi, I would like to study the effetc of temperature and humidity on the evolution of pheromone captures of a lepidopteran during one season. Indeed, I calculated correlation coefficient but I...

03 April 2017 322 4 View

How to deal with non normal data?

I'm working on 10 dependant variables, half of them have normal distribution but the other are not normally distributed so I transformed them by Log10 but only one variable still always non normal...

31 December 2016 3,557 15 View

Would Anova be helpful to study variation in temperatures in many years?

When we have temperatures in decades (ten days) for example for 5 years, could Anova be adequate to compare sums of tempreatures to know whether there is difference between these sums over the...

05 June 2016 9,356 5 View

What's the best time to collect plant parts for extraction?

To prepare plant extracts from leaves, fruits or the whole plant, what's the best time for collecting (morning, miday, ......etc)?

01 February 2016 8,869 7 View

How can I set up yellow water traps?

How are yellow water traps distributed in orchards or fields to capture insects : randomly or following a specific method to obtain good results?

31 December 2015 6,997 7 View

What are different methods of plant aqueous extraction?

I want to prepare aqueous extraction from plants and I need to know how to do it. Thank you for your contribution

04 May 2015 9,567 0 View

What's the best method to analyze the morality from data on the toxic effect of a product?

When we have data on the toxic effect of a product (pesticide or plant extract), how do you analyze this mortality (statistical test)?

02 March 2015 9,260 2 View

Is petroleum ether good for terpenes extraction?

I work on the toxicity of Euphorbiae sp against Blattella germanica using terpenes and I need to know if petroleum ether can help me to extract them.

01 February 2015 5,040 6 View

How to prepare and dry aqueous extraction?

In toxicity tests against insects and also molecules identification, I would like to know how to prepare aqueous extraction and analyze obtained extracts without altering chemical composition of...

01 January 1970 6,358 5 View

How to choose concentrations in a bioassay?

During tests of biopesticdes effects on insects in lab, what's the best method to determine concentrations interval?

01 January 1970 10,029 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View