Is there any scientific method for vitamin D depletion in rat? Could 4 weeks of sun deprivation plus poor vitamin D contented foods leads in vitamin D depletion?
Are you referring to sun deprivation in rats or humans?
To cause a depletion of vitamin D in rats is need to test various levels of dietary vitamin D(3) to achieve stable mean serum 25D levels ranging between 10 and 115 nM.