Can i use DTT during a Zn affinity chromatography?

I need to purify His6 tag containing protein and im using a Histrap that is been charged with Zn2+. Can i add DTT to my lysis buffer or the column will not tolerate it when i insert the lysate to...

12 October 2020 5,910 4 View

How can I solve a checkpoint file problem in Gromacs 2018.4?

Hello everyone, I am running a simulation for 500 ns but after 200 ns I get the following error: The input requested 100000000 steps, however the checkpoint file has already reached step...

16 January 2020 454 7 View

How to model Constant-Current Constant-Voltage Charging of Lithium-Ion Battery in Comsol?

I am trying to implement a constant-current constant-voltage charging strategy using the COMSOL standard lithium-ion battery model that comes with the chemical engineering module. The idea is to...

02 December 2019 9,563 3 View

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts)?

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts). Degassing takes place with 5 cycles of 1 minute degassing in vacuum and 1 minute...

18 November 2019 5,423 2 View

SEB or SEE for Jurkat and primary human/mice CD4+ T cell activation?

25 July 2017 338 7 View

How to check the observability of a LPV system?

Consider the discrete system x' = A(t)x + B(t)u y = C(t)x' + D(t)u The matrices A, B, C, D contain time-varying parameters (calculated by a second model) which are given in the initial state, but...

19 May 2017 2,813 1 View

How to detect low amounts of mRNA by qPCR?

I want to detect early expression of IL2 by qRT-PCR. I was not successful using random hexameric primers at the RT step and SYBR for detection. what can I do to improve my sensitivity? What...

01 February 2017 7,322 4 View

How do I calculate sensitivity of android acccelerometers?

Hello, I would like to make a sensitivity experiment on android built-in accelerometers (tri-axonal) an make a statistical analysis (correlation etc) in Matlab. My questions are: 1..What would be...

08 November 2016 9,127 1 View

Any suggestions for efficient Raji cell transfection?

Hi everyone. Has anyone worked with Raji cell transfection? I recently used AMAXA technology to transfect Raji cells with a CRISPR construct that resulted in a very poor efficiency (~1%). When I...

17 May 2016 5,476 4 View

Could Raman spectroscopy reveal the presence of Pt and Ru nanoparticles dispersed on a carbon powder?

09 November 2015 8,352 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

Treating Intermediate Variable in Linear Regression Analysis in SPSS?

While performing the Linear regression analysis in SPSS version 26.0.0. my intermediate/mediation variable goes to the excluded section. Please share your valuable suggestion "How to restrain the...

25 February 2021 4,591 5 View