23 June 2022 1 10K Report

Since N>300, can I ignore Shapiro Wilk < .00?

More Cheng Kim Bong's questions See All
Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Question about water vapor uptake of metal-organic frameworks?

I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...

24 July 2024 1,515 0 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

Is this a scam?

UK's Well-online Science Press sent an invitation to be a co-author and offers a payment of $30 per review.

22 July 2024 1,695 8 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

20 July 2024 4,951 1 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View

Why is serum-free media used when making cancer cell conditioned media?

I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...

11 July 2024 7,063 1 View

Stable cell line generation: GOI is detected by qPCR but protein is not expressed?

Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...

11 July 2024 4,812 5 View

NH3/NF3/H2 Remote plasma Oxi/nit selective etching mechanism help ??

Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...

11 July 2024 8,115 2 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

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hello every one. i have this output abt skewness and kurtosis, i confused bcs some articles said that we just look at value of skw and kr, but other said that shoul to see by z value (value skw or...

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Fully Connected Neural Network ?

How to modify a neuron in the fully connected layer individually and design it as a statistical indicator such as kurtosis or negative entropy，as shown in the figure

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Is the sediment also taken for percentile calculation for grain size analysis?

I am performing a graphical grain size analysis based on Folk and Ward (1957) and for that I require percentiles as specified in this article...

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What are skewness/kurtosis sampling distributions?

For the skewness and kurtosis coefficients sampling distributions, are they assumed to be normal or some other distribution shape?

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Is it possible for ITS regression to be used on individual level repeated cross sectional study database?

I am evaluating impacts of major health financing policy changes in Georgia (country). The database is household level and it is not panel data. Continues outcome variable is out-of-pocket health...

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Finding the differentially expressed genes?

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How to solve “excessive deformation” error while FSI simulation in carotid artery?

I have been trying for a long time to get my 2-way FSI using a Mechanical and fluent model of an patient-specific carotid artery to work but it keeps crashing with errors before finishing the...

28 February 2024 6,093 5 View

How does clock skew impact VLSI circuit performance, and what mitigation techniques are effective in minimizing its effects?

Clock skew in VLSI design refers to the variation in arrival times of clock signals at different parts of a chip. This can lead to timing issues and affect overall circuit performance....

02 January 2024 6,276 1 View

Absence of parsimonious solution in fsQCA?

I have a dataset (based on a 5-point Likert scale). Following the direct method, I first calibrated it as 4 (fully in), 3 (crossover point), and 2 (fully out), but given that it is skewed to the...

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