There are several providers of transcriptome library prep kits for sequencing on Illumina instruments, like:
- Illumina (TrueSeq Stranded mRNA Liprary Prep Kit)
- Kapa (Stranded RNA Seq Kit)
- NEB (NEBNext Ultra Directional RNA Library Prep Kit)
According to NEB's own advertising, the NEB kit has approx. twice the yield of Kapa, which again is clearly better than Illumina.
Have anyone compared these kits, both in yield and in easiness of handling/procedure? Any recommendations?
I just tried Illumina, so I cannot give you a detailed comparison. But I do know for sure that NEB is providing most of the reagents for Illumina, so what you get from them is basically the same thing, but cheaper and - if their advertising is correct - better.
I came across this journal that compares Illumina (TrueSeq) and NEB (NEBNext) for small RNA library preparation. It may not be fully relevant to your inquiry on transcriptome library preparation but I hope it will shed some light on it.
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