Considering the small size of the molecules, an association with bigger immunogenic protein is necessary (BSA, KLH, TT).
1- is a spacer necessary for a better recognition of the antigen (thinking of succinic acid)?
2- what are the best animal models to be chosen (mice, rabbits or others)?
3- After antibody purification, what is the stability of the retrieved antibodies? is it possible to use them for other detection methods (ELISA, WB etc)?
1. It usually ia advantageous to add a spacer. We have used from 2-4 glycine molecules.
2. That depends on the metabolic pathway, and which organs are metabolizing the compounds
3. Absolutely, the antibodies are usually very stable. They can last for years if properly treated and stored. aThe protocol used in my lab is to filter the antibodies and store them at -20C, with or without 10% (final concentration) of glycerin
Thanks for your answer, I would like to inquire more about the 3rd question because surprisingly enough, in our lab, we were confronted with an unusual situation.
Even though the antibodies were structurally intact, we had difficulties using them in WB. After some analysis we found out that the purified antibodies formed aggregates.
Any comment and suggestions about this situation are much appreciated.
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