The 'Biochemistry' of permed hair

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Predicting Depression Levels Using Social Media Posts

The use of Social Network Sites (SNS) is increasing nowadays especially by the younger generations. The availability of SNS allows users to express their interests, feelings and share daily...

01 January 1970 4,400 2 View

What is the role of asparagine in cancer?

Asparaginase is useful in the treatment of leukemia as it degrades asparagine. What role does asparagine play in the proliferation of cancer cells?

01 January 1970 4,720 3 View

Impact of the Covid-19 pandemic on the Aquaculture and Fisheries sector.

Hello all, With all the lockdown regulations, sales and trade may not be as it was before, goods and services are impacted; fish and fish products and services are not left out. Would you...

01 January 1970 3,700 13 View

The Role of Renin-Angiotensin-Aldosterone System in the Severity of COVID-19 in Diabetic/Hypertensive Patients

Hypertension, diabetes, and cardiovascular disease are the most prevalent comorbidities in patients with COVID-19. Although they do not appear to affect the infectivity of the virus, they do...

01 January 1970 8,859 8 View

'Biotechnology'- A textbook authored by V. Satyanarayana

Please who can help with copy of this textbook in PDF?

01 January 1970 5,980 5 View

Cancer cells and metastatic phenotype

Why do cancer cells use distinct transcriptomic and proteomic programs to reach the same metastatic phenotype?

01 January 1970 4,948 3 View

Biotechnology: Do the Benefits Outweigh the Downsides?

The major apprehension of genetic engineering is that through recombinant DNA experiments , unique micro-organisms or viruses (either inadvertently, or sometimes deliberately for the purpose of...

01 January 1970 965 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

The same lC-MS for Forensic Toxicological and Chemical expertises?

Dear Colleagues! How correct and appropriate is it to carry out a forensic toxicological examination of drugs and psychotropic substances in biological objects (in blood, urine, hair) and a...

24 January 2021 4,489 2 View