Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

Hi, I have a problem with my mouse Adipose MSC primary culture?

I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...

13 February 2021 9,838 1 View

How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

(Scale request) Anyone has the Malay version of Eating Attitude Test-26/40 (EAT-26/40)?

Dear Researchers, My research team is conducting a eating disorder related research in Malaysia and we would like to use the Malay translated version of EAT26/40. I have tried to email the...

24 January 2021 8,925 2 View

Can I make a meta-analysis with different variables in the same forest plot?

14 January 2021 1,733 4 View

Simultaneous imaging of multiple components (Carbohydrates, Fat, Protein) in food products using confocal microscopy??

Hi Everyone, I am trying to characterize the microstructure (localization of carbohydrates, fats, and proteins) of coconut milk powder using confocal microscopy. From the review of the available...

12 January 2021 5,464 2 View

Oil Red staining AML-12, How much time did you wait?

Hi, Was wondering if any of you conducted an Oil Red on AML-12 cells, if so, after how much time did you executed this? Some studies call for 24hr and some for 18hr. For fat content analysis....

03 January 2021 8,613 1 View

How to read an HPLC report?

I have four HPLC graphs (for methyl ethyl ketone (MEK) and sulfuric acid and little organic monomers) and I want to analyze them. Is there anyone who can help me with this?

23 December 2020 6,180 3 View

Does anybody face the wavy hair problem in C57BL/6 mice using High fat diet (60% calorie fat)?

10 December 2020 340 4 View

How to avoid bright non-specific labeling when doing iDISCO+ staining and light sheet imaging?

We routinely use the iDISCO+ and Adipoclear techniques followed by imaging on a light sheet system and I find very bright punctate labeling around the outside of tissues such as brain or fat as...

08 December 2020 1,675 1 View