Lymhpoma cell culture issues, help?

Hi! I need help for some issues in growing mantle cell lymphoma cell lines (REC-1, MAVER-1, Granta-519, Mino, Jeko-1). I noticed that it is a little bit hard to maintain a stable cell culture:...

09 February 2021 7,402 11 View

Help to set up an in vitro Bioaccessibility assay?

Hi everyone! i am looking for protocols to evaluate the bioaccessibility of carotenoids in plant matrix. Ideally the best would be a quite easy protocol that could be used massively in the lab,...

02 February 2021 5,516 2 View

How seminal was the influence of Giuseppe Campos Venuti and Federico Oliva in Italy, France, Spain and other countries?

The question is what remains in the urban planning and in our professional and intellectual experiences of Giuseppe Campos Venuti and Federico Oliva? What about discipline now? They reasoned and...

30 October 2020 5,450 4 View

Is Sytox (from Thermo Fisher Scientific) a good choice to dectect NETs in flow cytometry using whole blood?

I found several papers in the literature where Sytox (green, red, blue, or orange) is used to detect Neutrophil Extracellular Traps, but it's always used with purified neutrophils. Do you know if...

07 October 2020 5,865 2 View

HPLC Alternative for a new project?

dear collegues, i would like to know if there are any high throughput and specific techniques alternative to the HPLC analysis for protein detection and if possible their relative costs. Thank...

04 October 2020 9,928 3 View

Maximum concentration for HPLC???

Dear Collegues, I'm starting a new project and I need to know what is the maximum concentration for a sample that have to be processed by analytical HPLC. Thank you so much for the answers! Luca

01 October 2020 1,654 5 View

How to make ethyl cellulose water soluble?

Good evening, I'm looking for a way to make ethyl cellulose soluble in water. I read about a low ethoxylated formulation with a DS around 1-1,5 but I cannot find it in any shop. Is there anyone...

20 July 2020 8,592 1 View

Single cell RNA sequencing on immune cells infiltrated in tissues: what is the best way?

I am planning to investigate how immune cells infiltrated in certain tissues may change between different mouse models. If I don't want to focus on a specific immune cell type, is it possible to...

30 June 2020 1,834 3 View

Empatica E4 data processing tools and strategies?

Hello there! I am a Masters student working at EPFL with Empatica E4. For the moment, as both the device and the data from it are very new to me, I am testing the solidity of the signals. At first...

14 June 2020 6,185 2 View

Binary Classification - Machine Learning - When using which one?

Hey, I don't exactly understand how I make the decision to choose a SVM or a DNN or Linear Classifier for my binary classification problem. Are there any aspects to proove and then decide? Or is...

28 April 2020 4,728 4 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View